Relatively little is known about the life history of Cyprinella trichroistia (Tricolor Shiner), a species endemic to the Coosa and Cahaba rivers in Alabama, Georgia, and Tennessee above the Fall Line. The life history of this species was investigated using a series of collections obtained through the University of Alabama Ichthyological Collection spanning 27 months and collected from Hubbard Creek in the Coosa River System. A total of 6366 specimens were examined for age and growth, reproductive cycle, food habits, parasites, habitat, and associated species. Males and females have similar length-weight relationships, but males attain a larger size. Data analysis and field observations indicate that the species has a single reproductive season beginning in April and ending in August with peak activity in June. Numbers of mature ova of females ranged from 32 to 148, and was highly correlated to female size and weight. At any given time in the population, there were three (possibly four) age classes noted. The habitat for the Tricolor Shiner was primarily clear, flowing streams with a rocky substrate suitable for crevice spawning to occur. The diet consisted mainly of aquatic insects; the only parasites observed were internal nematodes and external trematodes.